In collaboration with Prof. Robert Marks and Prof. Aaron Lewis

 Taha, Marks, Gheber, Sukenik, Lewis, (2003), Appl. Phys. Lett. 83, 1041

We demonstrate the direct printing of proteins on a surface using a cantilevered nanopipette as the probe of a scanned probe microscope (Nano fountain pen - NFP). Proteins were directly delivered through the ~100 nm aperture of the nanopipette by simply contacting the probe with the surface, in ambient conditions. Protein features with dimensions as small as ~200 nm have been deposited and characterized both by Scanning Force Microscopy (SFM) and Near-field Scanning Optical Microscopy (NSOM). 

  BGU

Fluorescently labeled Avidin printed on an aldehyde slide

GFP dots printed on a BSA hardened layer

GFP line printed on a BSA hardened layer

 

NSOM: Topography of a dirty sample (left) and reflection at 488 nm (right), acquired simultaneously. Line is barely observable in topography, because of dust particles, but is the only feature that absorbs 488 nm in the right image, therefore is clearly visible.