|
- Development of experimental approaches using genetically engineered, cloned firefly luciferase as a tool to measure local ATP concentrations in intact cells and isolated organelles (yeast).
- Firefly luciferase mechanism: kinetics of light production.
- Cellular protein traffic using luciferase as a reporter for intracellular localization and assembly.
- Interaction of rat brain hexokinase with mitochondrial porin from various sources.
- Involvement of geometric and hydrophobic surface complementarity in macromolecular association.
- Characterization of an extracellular ATP receptor from frog respiratory epithelium.
- Molecular biology, towards transformation and genetic engineering of H. pluvialis, with emphasis on cloning, transformation and over-expression of heterologous genes, and use of firefly luciferase as a reporter gene;
- Cells fractionation and organelles (chloroplast and mitochondria) isolation, towards algal cellular biochemistry, including analysis of protein and enzymes levels and their coordination in anabolic pathways pertinent to the Unit’s interests (carotenoids, photosynthesis, and lipid synthesis);
- Experimental framework for the action and regulation of carotenoids biosynthetic pathway, and its relation with lipid synthesis, including quantitation of metabolic intermediates and fluxes under various physiological conditions
|