Eyal Nir-News


	July 2009 Roman: First single-molecules bursts observed in a newly build freely diffusing setup capable of measuring both FRET and ALEX ratios.
	March 2010 Roman: First time trajectories acquired from immobilized molecules by TIRF setup
	May 2010 Noa: DNA is doubly labeled with donor Cy3B and acceptor ATTO 647N through PCR with labeled primers.
	July 2010 Miran: First DNA Origami is visualized with AFM
	September 2010 Roman: First Fast dynamics of DNA hairpin was acquired with several millisecond time resolutions Rula: First demonstration of my motor activity: binding of walker and track followed by the releasing of the left-leg upon fuel addition.
	October 2010: Rula: Complete first step measurement: starting with constructing the complete motor (state-A), continuing with introducing first fuel and releasing of left-leg from T1 and binding to T3 (state-B).
	March 2011 Roman: More than 95% of DNA origami rectangle bound the surface in the desired orientation (face up) Tommy: First time successful measurement of walker striding on origami on the TIRF setup. Noa: Reconstitution of nucleosome from 4 histones and doubly labeled DNA. Noa: Doubly-labeled (dl) DNA with H4-E63C-ATTO 647N octamer. 1. Only doubly-labeled DNA. 2. NCP with dlDNA and octamer without H4-ATTO 647N. 3. NCP with dlDNA and octamer containing one copy of H4-ATTO 647N. 4. NCP with dlDNA and octamer containing two copies of H4-ATTO 647N.
	April 2011: Rula: Three motor states (A, B and C) detected and side reactions are identified. Tommy: A successful completion of full striding cycle of walker on origami.
	July-August 2011: Miran: managed to receive good separation of DNA Origami-AuNP complexes form unbonded DNA Origami and free AuNP.
	September 2011: Congratulation to Tommy. His paper titled “Disentangling subpopulations in single-molecule FRET and ALEX experiments with photon distribution analysis” was accepted to Biophysical Journal September-October 2011: Rula: Successful measurement of the kinetics of binding walker to track and of leg-lifting reaction upon fuel addition.