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July
2009
Roman: First
single-molecules
bursts observed in a newly build freely diffusing setup capable of
measuring
both FRET and ALEX ratios.
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March
2010
Roman: First
time trajectories
acquired from immobilized molecules by TIRF setup
![TIRF](images/Results/New%20News/Roman2.jpg)
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May
2010
Noa: DNA is doubly labeled
with donor Cy3B and acceptor ATTO 647N
through PCR with labeled primers.
![Doubly Labeled DNA](images/Results/New%20News/Noa1.jpg)
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July
2010
Miran: First DNA Origami is
visualized with AFM
![Origami on AFM](images/Results/New%20News/Miran1.jpg) |
September
2010
Roman:
First
Fast dynamics of DNA hairpin was acquired with several millisecond time
resolutions
Rula: First demonstration
of my motor activity: binding
of
walker and track followed by the releasing of the left-leg upon fuel addition.
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October
2010:
Rula: Complete first step
measurement: starting with
constructing the complete motor (state-A), continuing with introducing
first
fuel and releasing of left-leg
from T1 and binding to T3
(state-B).
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March
2011
Roman: More than 95% of
DNA origami rectangle bound the surface in the desired orientation
(face up)
![Origami Orientation](images/Results/New%20News/Roman4.jpg)
Tommy: First time
successful measurement of walker striding on origami on the TIRF setup.
![Walker in FRET](images/Results/New%20News/Tommy3.jpg)
Noa: Reconstitution
of nucleosome from 4 histones and doubly labeled DNA.
![Histones and DNA](images/Results/New%20News/Noa2.jpg)
Noa: Doubly-labeled
(dl) DNA with H4-E63C-ATTO 647N
octamer.
1.
Only
doubly-labeled DNA.
2. NCP with
dlDNA and octamer without H4-ATTO 647N.
3. NCP with
dlDNA and octamer containing one copy of H4-ATTO 647N.
4. NCP with
dlDNA and octamer containing two copies of H4-ATTO 647N.
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April
2011:
Rula: Three
motor states (A, B and C) detected and side
reactions are identified.
![Motor's States](images/Results/Research/Rula1.jpg)
Tommy: A
successful completion of full striding cycle of walker on origami.
![Walker Striding](images/Results/New%20News/Tommy2.jpg)
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July-August 2011:
Miran: managed to receive good
separation
of DNA Origami-AuNP complexes form unbonded DNA Origami and free AuNP.
![DNA Origami-AuNP Complexes](images/Results/New%20News/Miran2.jpg)
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September
2011:
Congratulation
to Tommy. His paper titled “Disentangling subpopulations in single-molecule FRET and ALEX experiments with photon
distribution analysis” was accepted to
Biophysical Journal
![Photon Distribution Analysis](images/Results/New%20News/Tommy1.jpg)
September-October
2011:
Rula: Successful
measurement of the kinetics of binding
walker to track and of leg-lifting reaction upon fuel addition.
![Kinetics](images/Results/New%20News/Rula1.jpg)
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