Cloning of cyt1Aa with and without p20 into the expression vectors pHE4-A, pHE4-D and pHE4-AD harboring cry4Aa and cry11Aa. P/O indicate early T7 promoter PA1, which utilizes the usual E. coli RNA polymerase and two tandem lacO operators. (From: Khasdan et al., Environ. Microbiol. 3: 798-806, 2001).

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